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Job Details

Sequencing Technician

Location
San Francisco, CA, United States

Posted on
Oct 03, 2020

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:,ActualValueFromSolar:null},{QuestionName:Job Summary,AnswerValue:JOB OVERVIEWu003cbru003eu003cfont style=color:undefined;font-family:helvetica,sans-serif;size:undefined;\u003eAs the HiSeq/NovaSeq operator, the Sequencing Technician is responsible for several aspects of DNA sequencing.u0026nbsp; This starts with answering questions about sequencing projects, taking in libraries and submission forms, and checking them for completeness.u0026nbsp; Once samples have been submitted to the CAT, the operator will update the CAT website containing the sample queue and run calendar.u0026nbsp; u003c/fontu003eu003cbru003eu0026nbsp;u003cbru003eu003cfont style=color:undefined;font-family:helvetica,sans-serif;size:undefined;\u003eWhen enough samples are ready to be processed, the operator will quantify the libraries by qPCR, Qubit, or digital PCR and calculate the molar concentration.u0026nbsp; With this information, the operator will run the samples on the HiSeq/NovaSeq.u0026nbsp; Following runs, the operator will verify proper run performance, take care of the appropriate post run maintenance, and demultiplex the data.u0026nbsp; After transferring the data to the CAT server, the operator will notify users that their data is ready for download and answer any questions they have.u0026nbsp; The operator will also be in charge of keeping an inventory of supplies and ordering more when necessary.u003c/fontu003eu003cbru003eu0026nbsp;u003cbru003eu003cfont style=color:undefined;font-family:helvetica,sans-serif;size:undefined;\u003eThe Sequencing Technician will train CAT users on the equipment in the CAT, answer questions regarding use, and contact service engineers if necessary. u003c/fontu003eu003cbru003eu0026nbsp;u003cbru003eu003cfont style=color:undefined;font-family:helvetica,sans-serif;size:undefined;\u003eThe Sequencing Technician will u003c/fontu003eu003cspan style=background:white;\u003eu003cspan style=color:#333333;\u003eu003cfont style=color:undefined;font-family:helvetica neue;size:undefined;\u003eu003cfont style=font-size:10.5pt;\u003econduct research developing new genomic methods, often in collaboration with different groups on campus or external partners.u0026nbsp;u003c/fontu003eu003c/fontu003eu003c/spanu003eu003c/spanu003eu003cbru003eu0026nbsp;u003cbru003eu003cfont style=color:undefined;font-family:helvetica,sans-serif;size:undefined;\u003eBIOCHEMISTRY u0026amp; BIOPHYSICSu003c/fontu003eu003cbru003eu003cfont style=color:undefined;font-family:helvetica,sans-serif;size:undefined;\u003eu003cfont style=font-size:10.0pt;\u003eThe Department of Biochemistry u0026amp; Biophysics is a large teaching and research department within the School of Medicine (SOM), and is comprised of 27 primary faculty, 25 joint faculty, 58 non-faculty academic staff, 85 postdoctoral fellows, about 120 graduate students, and 100 non-academic staff.u003c/fontu003eu003c/fontu003eu003cbru003eu003cbru003eABOUT UCSFu003cbru003eThe University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and excellence in patient care. It is the only campus in the 10-campus UC system dedicated exclusively to the health sciences.,VerityZone:jobdescription,QuestionType:textarea,ActualValueFromSolar:null},{QuestionName:Organization,AnswerValue:Campus,VerityZone:location,QuestionType:select,ActualValueFromSolar:null},{QuestionName:Req Number,AnswerValue:55911BR,VerityZone:autoreq,QuestionType:text,ActualValueFromSolar:null},{QuestionName:Location,AnswerValue:Mission Bay,VerityZone:formtext8,QuestionType:single-select,ActualValueFromSolar:null},{QuestionName:Job Title,AnswerValue:Sequencing Technician,VerityZone:formtext10,QuestionType:text,ActualValueFromSolar:null},{QuestionName:Employee Class,AnswerValue:Career,VerityZone:formtext12,QuestionType:radio,ActualValueFromSolar:null},{QuestionName:Percentage,AnswerValue:100%,VerityZone:formtext13,QuestionType:Text,ActualValueFromSolar:null},{QuestionName:Shift Length,AnswerValue:8 Hours ,VerityZone:formtext14,QuestionType:Radio,ActualValueFromSolar:null},{QuestionName:Shift,AnswerValue:Days,VerityZone:formtext15,QuestionType:Checkbox,ActualValueFromSolar:null},{QuestionName:Work Days,AnswerValue:Monday - Friday, 40 hours/week,VerityZone:formtext16,QuestionType:Text,ActualValueFromSolar:null},{QuestionName:Required Qualifications,AnswerValue:• BS/BA degree in Biology, Chemistry, Physics, Engineering, or Biochemistry and one or more years of recent, directly related work experience following degree completion, utilizing the techniques or methods required by the position; or an equivalent combination of education and experienceu003cbru003e• At least one year of experience working in a labu003cbru003e• Demonstrate practical knowledge of Next Generation Sequencing sample preparation workflows and applicationsu003cbru003e• Ability to make analytical calculations efficiently (molarity, dilution calculations)u003cbru003e• Able to perform basic molecular biology techniques including DNA cloning, PCR, gel electrophoresisu003cbru003e• Aptitude for technology and instrumentationu003cbru003e• Effective communication skills,VerityZone:formtext3,QuestionType:textarea,ActualValueFromSolar:null},{QuestionName:Preferred Qualifications,AnswerValue:• One year of experience performing researchu003cbru003e• Experience with DNA sequencingu003cbru003e• Experience with qPCRu003cbru003e• Knowledge and experience with NGS data analysis (demultiplexing, bioinformatics)u003cbru003e• Experience and demonstrated proficiency with scripting and operating automated liquid handling systems,VerityZone:formtext4,QuestionType:textarea,ActualValueFromSolar:null},{QuestionName:License/Certification,AnswerValue:n/a,VerityZone:formtext5,QuestionType:textarea,ActualValueFromSolar:null},{QuestionName:Position Type,AnswerValue:Full Time,VerityZone:formtext6,QuestionType:radio,ActualValueFromSolar:null},{QuestionName:Department Name,AnswerValue:Biochemistry and Biophysics,VerityZone:formtext17,QuestionType:text,ActualValueFromSolar:null},{QuestionName:Equal Employment Opportunity,AnswerValue:The University of California San Francisco is an Equal Opportunity/Affirmative Action Employer. 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